Figure 6.

L1CAM+ EVs contain a distinct and interesting protein signature. Total EVs were isolated from plasma from four healthy volunteers and subsequently immunoprecipitated with L1CAM or EpCAM antibodies. The samples from the four subjects were then pooled to generate a sufficient amount of protein for five membrane antibody arrays [Human Obesity Antibody Array (A), Human Phospho-Kinase Antibody Array (B), MAPK Antibody Array (C), Human Kidney Biomarker Antibody Array (D), and Human Apoptosis Antibody Array (E)]. An equivalent amount of protein per sample type was loaded to each spot. The levels of different proteins were calculated by densitometric analysis and normalized to the reference proteins present on each membrane. Results are depicted as heatmaps ranging from zero to maximum value and scaled according to the color bars.