Table 1.

Comparison of different methods for isolating axonal miRNAs.

Method	Advantage	Disadvantage	Optimal application
Campenot chamber	High purity, good for fluidic separation	Not easily manageable, low yield of axonal RNA	Cultured cells
Boyden chamber	Easily manageable, high yield of RNA	Not good for fluidic separation and live cell imaging	Cultured explants/cells
Microfluidic chamber	Easily manageable, high purity, very good for fluidic separation, good for live cell imaging	Low yield of axonal RNA	Cultured cells
Axoplasmic isolation	High yield of axonal RNA, good in vivo system	Not easily manageable	In vivo preparation of RNA granules