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. Author manuscript; available in PMC: 2017 May 22.
Published in final edited form as: J Alzheimers Dis. 2016;51(1):107–121. doi: 10.3233/JAD-151039

Fig. 1.

Fig. 1

Hemin induces endocytosis of PrPC. SH-SY5Y and K562 cells expressing PrPC-GFP or PrPΔ51-89-GFP were exposed to vehicle (left panels) or 30 μM hemin (right panels), and images of live cells were captured after 30 minutes. Hemin induces endocytosis of PrPC-GFP in both SH-SY5Y and K562 cells (panel 1 & 5 versus 2 & 6). In SH-SY5Y cells PrPC-GFP accumulates in intracellular endocytic vesicles (panel 2), while in K562 cells expression on the plasma membrane decreases without accumulation in endosomes. A small amount of PrPC-GFP and PrPΔ51-89-GFP are detected in the perinuclear region in the Golgi compartment (panel 6). Hemin has no effect on the distribution of PrPΔ51-89-GFP in either cell line (panels 3 & 7 versus 4 & 8). Bar: 10 μm.