Fig. 4.

α- and α-globin expression is reduced in RBCs from PrP^−/− mice. A, B) Lysates of washed RBCs from PrP^+/+ and PrP^−/− mice reveal reduced expression of α-globin (A) and β-globin (B) in PrP^−/− relative to PrP^+/+ samples (lanes 1–3 versus 4–6). Dimeric forms of α-globin and β-globin are evident after a longer exposure (right panels). C, D) Densitometry confirms significant reduction of α-globin and β-globin in PrP^−/− samples relative to PrP^+/+ controls. Reaction for β-actin provides a loading control. Data are Mean ± SEM of three independent experiments on multiple mice. ^*p < 0.05, ^**p < 0.01.