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. Author manuscript; available in PMC: 2017 May 22.
Published in final edited form as: J Alzheimers Dis. 2016;51(1):107–121. doi: 10.3233/JAD-151039

Fig. 7.

Fig. 7

Hemin induces α-globin expression in mouse brain organotypic cultures. Lysates of organotypic brain cultures from PrP+/+ and PrP−/− mice cultured in the absence or presence of hemin were processed for western blotting. Probing for α-globin reveals significantly more α-globin in hemin treated PrP+/+ relative to PrP−/− samples (lanes 1 & 2 versus 3 & 4). Human brain sample fractionated in parallel shows significantly more α-globin relative to mouse samples (lanes 1–4 versus 5), and slower migrating forms that are absent in mouse brain and RBC lysates (lane 5, *). Expression of PrPC is as expected (lanes 1–5). Reaction for β-actin provides a loading control.