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. Author manuscript; available in PMC: 2018 Feb 20.
Published in final edited form as: Chem Res Toxicol. 2016 Dec 15;30(2):657–668. doi: 10.1021/acs.chemrestox.6b00394

Figure 3.

Figure 3

Identification and characterization of N2-Acetyl-AαC-6-O-Gluc. (A) LC/MS3 product ion mass spectra of N2-Acetyl-AαC-6-O-Gluc (m/z 418.1 > 242.1 >) formed in primary human hepatocytes. N2-Acetyl-AαC-6-O-Gluc was purified by HPLC and characterized by mass spectrometry. (B) UV spectra of AαC-6-OH, AαC-3-OH, N2-acetyl-AαC-O-Gluc and its hydrolysis product obtained after β-glucuronidase followed by acid treatment of N2-acetyl-AαC-O-Gluc as described in material and method.