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Reactivity of the anti-phospho-S116 eNOS antibody with ERK2-phosphorylated and nonphosphorylated mouse and bovine eNOS proteins. Purified wild-type mouse or bovine eNOS was incubated with purified constitutively active ERK2 for 1 h at 30 °C under the conditions described in Materials and methods. Results shown are representative of 3 separate experiments.
