Table 1.
Antibodies Used for Immunofluorescence
Antigen | Blocking serum | Primary antibody | Secondary antibody |
---|---|---|---|
Collagen type I A1 | Mouse serum | Rabbit polyclonal (1:80 dilution) | Mouse antirabbit IgG-FITC (1:100 dilution) |
Osteocalcin | Donkey serum | Goat polyclonal (1:50 dilution) | Donkey antigoat IgG-PE (1:100 dilution) |