Figure 6.
PEITC administration decreases fatty acid metabolite levels in the prostate adenocarcinoma of TRAMP mice. (A) Levels of total free fatty acids, total phospholipids, triglyceride, and ATP in prostate tumors of control and PEITC-treated TRAMP mice. Results shown are mean ± SD (n = 8 for control and n = 4 for PEITC treatment group). Tumor tissues from different mice of each group were used for the assays. Statistical significance was determined by unpaired Student’s t test. (B) Representative images for BODIPY493/503 staining showing lipid droplets in prostate adenocarcinoma of a control and a PEITC-treated TRAMP mouse. (C) Quantitation of number of lipid droplets/cell (60x magnification, scale bar = 5 μm). Results shown are mean ± SD. Statistical significance was determined by unpaired Student’s t test. (D) A simplified cartoon for fatty acid synthesis. PEITC-mediated suppression of fatty acid metabolism proteins and intermediates is also summarized. Abbreviations: FACS- fatty acyl-CoA synthase; ACLY- ATP citrate lyase; ACC- acetyl-CoA carboxylase; FASN- fatty acid synthase; and CPT- carnitine palmitoyltransferase.