Fig 6. Autophagy inhibitor facilitates Ge induced cell death via mitochondrial apoptosis pathway.

The above cells were treated with DMSO, 3-MA, Baf.A, Ge, Ge+3-MA and Ge+Baf.A for 48 hours and were subjected to western blot using following antibodies, Cytochrome C (Cyto C), cleaved CASP3, BAX, Bcl-2 and ACTB (A). Hematoxylin-eosin staining (B) and immunohistochemical assays (anti-cleaved CASP 3) (C) were performed in MDA-MB-468 xenograft.