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. 2017 May 22;12(5):e0178103. doi: 10.1371/journal.pone.0178103

Fig 3. Psychosine reduces sphingomyelin lateral mobility.

Fig 3

A-C) Delayed time to recover from photobleaching by BODIPY-SM (A), BODIPY-PC (B) and BODIPY-GM1 (C) (N = 61–648 in 1–4 independent experiments). D-F) The mobile fraction of BODIPY-SM (D) but not that of BODIPY-PC (E) and BODIPY-GM1 (F) in Twitcher and wild-type RBCs treated with psychosine were significantly lower than wild-type RBCs. G-I) Similarly, the half-life of FRAP signal was increased for BODIPY-SM (G) but not for BODIPY-PC (H) and BODIPY-GM1 (I) in Twitcher and wild-type RBCs treated with psychosine, evidencing an effect of psychosine in the plasma membrane and an interaction with sphingomyelin. All aforementioned psychosine incubations were 30 minutes at 37°C Results are mean ± SEM. NS, not significant (>0.05); *, p <0.05; **, p <0.01; ***, p <0.001.