Table 3.
Transfection conditions and targeting efficiency for loci not expressed in undifferentiated hESCs.
| Targeted locus* | YearREF | Electroporation settings | AntibioticR colonies/starting cell no. | No. of properly targeted colonies/total | Positive selection | Negative selection | Homology arms 5′/3′ (Kb) |
|---|---|---|---|---|---|---|---|
| MIXL1 | 2008 9 | Data not provided | Data not provided | Data not provided | PGK‐Neo | 9.4/1.9 | |
| FEZF2 | 2009 18 | 320V 200 μF | 128 NeoR/1.5 × 107 145 NeoR/1.05 × 107 | 2/675 (0.3%) 2/130(01.5%) | PGK‐Neo | – TK | 4.2/4.1 4.2/4.1 |
| OLIG2 | 2009 25 | 250V 250 μF | 106 NeoR/0.5–1 × 107 | 6/106 (5.7%) | PGK‐Neo | TK | 2/5.3 |
| ISL1 | 2009 16 | 320V 200 μF | Data not provided | Data not provided | Puro | 9.2/5.5 |