FIG. 2.

Mapping of the filamin A-interacting domain of PEBP2β by yeast two-hybrid analysis. (A) Schematic diagram of domain structures found in the PEBP2β protein and description of carboxy-terminal deletion mutants of PEBP2β. α, β, and L represent α helixes, β strands, and loops, respectively. The thick lines at the top indicate areas I and II of the Runt-interacting region (30). (B) PEBP2β and its deletion mutants were tested for the ability to interact with filamin A-C. Yeast cells were transformed with the indicated combinations of plasmids and grown on nonselective (−Trp, −Leu) and selective (−Trp, −Leu, −His, 1 mM 3-amino-1,2,4-triazole) media. Lamin/large T antigen and Runx1/PEBP2β served as negative and positive controls, respectively.