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Effect of PEG 2K incubation time on the RNA and PEG loading of RNA–SNAs. (A) Antisense and sense RNA loading does not change significantly with increasing incubation time with PEG 2K; however, the loading of PEG does increase with longer incubation times. (B) The increase in PEG loading corresponds to an increase in the hydrodynamic diameter and the zeta potential of SNAs.
