Skip to main content
. 2017 May 1;10:121. doi: 10.3389/fnmol.2017.00121

Figure 5.

Figure 5

Ankfy1/+ mice with normal myelin sheaths show reduced neurotrophic factor (NTF) mRNA expression. (A) Eriochrome cyanine staining reveals similarly sized myelin sheaths in the corpus callosum, cerebellum and pons of Ankfy1/+ mice at 4 months of age. The scale bar represents 300 μm. (B) Quantitative assessment of the thickness of the corpus callosum and cerebellar medulla per image field (n = 12). (C) RT-qPCR was used to determine the levels of the myelin basic protein (MBP) and retinoic acid receptor-related orphan receptor α (RORα) mRNAs in the cerebellum of P21 and P30 WT and Ankfy1/+ mice. The relative abundance of the MBP mRNA was calculated by setting the value obtained for the control mice to 1, and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) served as an internal control. (D) Brain-derived neurotrophic factor (BDNF), mesencephalic astrocyte-derived neurotrophic factor (MANF) and neurotrophin-3 (NT-3) mRNA expression levels in the cerebellum of WT and Ankfy1/+ mice were analyzed using RT-qPCR. The mRNA expression levels were normalized to the GAPDH mRNA expression levels (n = 4, *p < 0.05, **p < 0.01, ***p < 0.001). The data represent means ± SEM.