FIG. 2.

The expression of POT1-RNAi caused the loss of telomeric overhang signals. (A) T-OLAs were performed with oligonucleotides complementary to the G-rich strand [CCCTAA]₄, the C-rich strand [TTAGGG]₄, or the mismatch sequence [CCCTTA]₄. (B) T-OLA analysis was used on DNA derived from IMR90 cells expressing POT1-siRNA and controls at 3, 5, and 7 days after transfection. Quantitative PCR was performed using primers specific for genomic GAPDH for equal amounts of genomic DNA in each sample (bottom). (C) The in-gel hybridization assay was performed with a native gel and probed with [CCCTTA]₄. DNA was derived from IMR90 cells expressing POT1-RNAi. (D) The DNA was denatured in the gels and rehybridized with the same probes. (E) Quantitative data of the loss of telomeric overhangs were derived from three independent experiments similar to those shown in panel C, and the average value (error bars represent standard deviations) was plotted.