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. 2005 Feb;25(3):1070–1080. doi: 10.1128/MCB.25.3.1070-1080.2005

FIG. 3.

FIG. 3.

POT1-RNAi induced the DNA damage pathway, apoptosis, chromosomal abnormalities, and the senescent phenotype. (A) The time course of Western blotting analysis in MCF-7 cells expressing POT1-siRNA (POT1-A) or lentivirus POT1-shRNA (sh1). A random sequence siRNA was used as a control. (B) TUNEL assay of MCF7 cells after expression with POT1-siRNAs or shRNAs. A random sequence siRNA was used as a control. (C) Growth curve of IMR90 cells expressing lentivirus POT1-shRNAs and vector control cells. (D) The induction of chromosomal abnormalities. IMR90 cells were transduced with indicated siRNA or shRNA for 7 days, and the chromosome was prepared and analyzed as described in Materials and Methods. Open arrow, chromosome break; solid arrow, dicentric chromosome. (E) POT1-RNAi induced the senescent phenotype. IMR90 cells were stained for β-Gal activity at pH 6.0. Graphs show the effect of expression of POT1-siRNAs and shRNAs.