FIG. 5.
(A) POT1 colocalized with endogenous TRF1 and TRF2 in IMR90 fibroblasts by immunostaining with anti-TRF1, anti-TRF2, and anti-POT1 antibodies. POT1 reduced the colocalization with TRF1 2 days after expressing TRF2ΔBΔM or TRF2-siRNA. (B) POT1 associated with telomeric DNA. ChIP of HT1080 cells expressing pLPC-Myc, Myc-POT1, Myc-TRF2ΔBΔM, or POT1-A-siRNA with indicated antibodies or preimmune serum (PI) of POT1 antibody for 2 days after infection. Duplicate dot blots were probed for telomeric or Alu repeats. (C) The quantification of the data in panel B representing the percentage of TTAGGG DNA recovered in each sample. Averaged signals obtained with total DNA samples were used as 100% value for the quantification.