TABLE 1.
Induction of chromosomal abnormalities in IMR90 cells
| Expressed siRNA or genes | Mean abnormalities/ cell (SD)a
|
% of cells with abnormalities (SD) | P valuec | |
|---|---|---|---|---|
| Fusionb | Breaks | |||
| Control | 0 | 0.03 (0.05) | 3 (5) | |
| siPOT1-A | 0.04 (0.06) | 0.29 (0.18) | 25 (16) | 0.06 |
| siPOT1-B | 0.02 (0.02) | 0.20 (0.1) | 20 (9) | 0.03 |
| POT1-sh1 | 0.10 (0.08) | 0.30 (0.1) | 30 (12) | 0.03 |
| POT1-sh2 | 0.08 (0.06) | 0.30 (0.1) | 28 (9) | 0.03 |
| TRF2ΔBΔM | 0.40 (0.15) | 0.62 (0.66) | 43 (31)d | 0.02 |
| TRF2ΔBΔM+POT1 | 0 | 0.17 (0.07) | 17 (7) | 0.05 |
| POT1 | 0 | 0.04 (0.01) | 4 (1) | 1.0 |
a
The numbers indicated average abnormalities per metaphase cell from four independent experiments; a total of 100 cells were scored.
b
Chromosome fusion contained multicentric chromosomes and chromosome end joining.
c
Frequencies of percent of cells with abnormalities were compared with the control group.
d
Frequency was higher than the TRF2ΔBΔM + POT1 group with a P value of 0.08.