Figure 2.

Overload increases skeletal muscle glucose uptake via a facilitated GLUT and not an SGLT. WT CD-1 female mice underwent unilateral synergist ablation surgery to induce plantaris muscle hypertrophy. After 5 days, muscles were excised and preincubated in KRBB for 30 min. A: For SGLT inhibition, muscles were incubated with phloridzin (10 or 100 μmol/L) or its vehicle (0.1% EtOH) for 60 min prior to the assessment of [³H]-2-deoxy-d-glucose uptake for 10 min. B: For GLUT inhibition, muscles were incubated in KRBB for 60 min and then simultaneously incubated with [³H]-2-deoxy-d-glucose and either cytochalasin B (10 μmol/L) or its vehicle (0.2% DMSO) for 10 min. The entire muscle incubation period for both inhibitor studies was 100 min. Statistical significance was defined as P < 0.05 and denoted as follows: a, vs. sham; b, vs. vehicle (either DMSO or EtOH). N = 4–6 muscles/group.