Table 2.
mGLUT4 KO mice do not exhibit impairments in skeletal muscle growth
| Genotype | WT/CON | mGLUT4 HET | mGLUT4 KO |
|---|---|---|---|
| Presurgery body weight | |||
| Fed state (g) | 22.6 ± 0.5 | 23.9 ± 0.7 | 21.3 ± 0.6b |
| Body composition | |||
| Fat mass (g) | 3.1 ± 0.3 | 3.9 ± 0.4 | 3.1 ± 0.3 |
| Lean mass (g) | 16.8 ± 0.3 | 17.5 ± 0.4 | 15.9 ± 0.4 |
| Fat mass (%) | 14.2 ± 1.0 | 16.1 ± 1.4 | 14.2 ± 1.3 |
| Lean mass (%) | 74.6 ± 1.1 | 73.5 ± 1.4 | 74.6 ± 1.2 |
| Pre–tissue collection body weight | |||
| Fasted state (g) | 20.5 ± 0.4 | 21.3 ± 0.5 | 19.0 ± 0.6b |
| Plantaris muscle weight | |||
| Sham (mg) | 12.5 ± 0.3 | 12.4 ± 0.3 | 12.5 ± 0.4 |
| Overload (mg) | 18.8 ± 0.4a | 18.6 ± 0.7a | 17.5 ± 0.8a |
| Plantaris muscle weight/body | |||
| Sham (mg/g) | 0.61 ± 0.02 | 0.59 ± 0.02 | 0.66 ± 0.02 |
| Overload (mg/g) | 0.93 ± 0.04a | 0.89 ± 0.02a | 0.93 ± 0.05a |
| Percentage change in muscle weight (%) | 50.9 ± 4.0 | 50.9 ± 4.6 | 40.1 ± 4.9 |
Body weight and body composition were examined in female WT/control (WT/CON: GLUT4 LoxP+/−, GLUT4 LoxP+/+, and Cre+), mGLUT4 HET, and mGLUT4 KO mice at 11–12 weeks old. Plantaris muscle hypertrophy was induced by unilateral synergist ablation of the distal two-thirds of the gastrocnemius and soleus muscles. The contralateral limb was sham operated and served as the control. After 5 days, plantaris muscles were excised and weighed. Statistical significance was defined as P < 0.05 and denoted as follows: avs. sham-operated controls, bvs. mGLUT4 HET mice. N = 8–15 mice or muscles/group.