Figure 1.

Islet phospholipid AA and EPA contents are reflective of diet and can be changed in vivo. Islets were isolated from 10-week-old BTBR-WT and BTBR-Ob mice and subjected to lipid extraction followed by thin-layer chromatography to isolate the phospholipid fraction for fatty acids species determination by gas chromatography. AA and EPA are displayed as a percent of total fatty acids (A) or the ratio between the two (B). Data were compared by unpaired t test within each species (n = 11–15). C: Fatty acid analysis as described above was performed with islets from BTBR-WT mice fed a chemically defined control (Ctrl) or EPA-enriched diet for 6 weeks after weaning. N = 5–7; ****P < 0.0001. D–H: BTBR-WT and BTBR-Ob mice were fed a chow diet or an AIN-93g–based control, EPA-enriched, or AA-enriched diet for 6 weeks upon weaning. Random-fed blood glucose (D), weight (E and F), and food intake (G and H) were measured weekly starting the Monday after the diet was started (3–6 days after diet start). Data were analyzed by two-way ANOVA with a Tukey posttest (n = 2–6). ND, not detected. *P < 0.05, **P < 0.01, ***P < 0.001 compared with Chow OB; †P < 0.05 compared with Ctrl OB.