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. 2017 Mar 7;66(6):1611–1625. doi: 10.2337/db16-1079

Figure 6.

Figure 6

Increased beige/thermogenic gene expression in the iWAT Id1−/− mice. A: Immunofluorescence staining showing the localization pattern of CD45 and Id1 in the iWAT of 2-month-old wild-type mice. iWAT sections incubated only with fluorescent secondary antibodies served as negative (–ve) controls. Scale bars = 100 μm. B and C: Relative mRNA transcript levels of beige and thermogenic genes in the iWAT of Id1+/+ and Id1−/− male mice after exposure to 4°C for 12 h (n = 7). D: Representative immunohistochemistry staining showing the expression pattern of UCP1 in the iWAT of Id1+/+ and Id1−/− mice after exposure to 4°C for 12 h (n = 5; 3 of 5 Id1−/− mice showed strong UCP1 staining in the iWAT; no staining was detected in the Id1+/+ mice). Scale bar = 10 μm. iWAT sections incubated only with horseradish peroxidase–conjugated secondary antibody served as the –ve control. Data are mean ± SD. *P < 0.05.