Table 1. Preimplantation development of the zygotes injected with mRNA at various concentrations.
| Concentration of mRNA | 1 cell (%) (12 hpi) | 2 cell (%) (24 hpi) | 4–8 cell (%) (48 hpi) | Mor. (%) (72 hpi) | Blast. (%) (96 hpi) |
|---|---|---|---|---|---|
| Intact | 72 (100) | 70 (97.2) | 66 (91.7) | 68 (94.4)* | 66 (91.7) |
| 50 | 63 (100) | 63 (100) | 62 (98.4) | 61 (96.8) | 57 (90.5) |
| 100 | 70 (100) | 70 (100) | 67 (95.7) | 67 (95.7) | 63 (90.0) |
| 250 | 72 (100) | 71 (98.6) | 64 (88.9) | 64 (88.9) | 58 (80.6) |
| 500 | 71 (100) | 71 (100) | 67 (94.4) | 66 (93.0) | 59 (83.1) |
*: At 72 hpi, the embryos developed to morula stage