Fig 7. Effects of STS on intracellular Ca²⁺ in mouse airway epithelial cells.

(A) Representative intracellular Ca²⁺ transients elicited by STS (10 μM) detected by fluo-3 fluorescence in the presence or absence of 2.8 μM atropine or in extracellular Ca²⁺ free solution. (B) Statistical analysis of intracellular Ca²⁺ in response to STS in N-PSS (control, 10 μM, n = 13) or in Ca²⁺ free PSS (n = 5) and the inhibitory effects of atropine (2.8 μM, n = 12), U73122 (20 μM, n = 3), 2-APB (50 μM, n = 6), Tg (10 μM, n = 7) and CPZ (10 μM, n = 3) on the Ca²⁺ transients elicited by STS (10 μM). Data is expressed as a percentage of the initial fluo-3 fluorescence. Values are mean ± S.E.M. (ANOVA, *P < 0.05 compared with the control).