FIG. 3.

Transcriptional activity and large-scale chromatin decondensation activity of inducible acidic activators. (A) The transient transcription assay used an 8- or 256-lac operator-E1b TATA-luciferase reporter plasmid in CHO-K1 cells. Fold induction is shown, meaning activity in the presence of rapamycin divided by activity in the absence of rapamycin. (B) Recruitment of each FRB*-YFP fusion protein to the chromatin array; see Materials and Methods for details. The median is shown. (C) Chromatin-unfolding assay. Chromatin arrays were measured for each FRB*-YFP fusion protein in the presence and absence of rapamycin. CFP-lac rep-FKBP3 was cotransfected with all FRB*-YFP fusion proteins. Tails of the box plots mark the 5th and 95th percentiles, boxes mark the 25th and 75th percentiles, and the line in the box marks the median for each sample. See Materials and Methods for details.