Skip to main content
. Author manuscript; available in PMC: 2017 Oct 14.
Published in final edited form as: RSC Adv. 2016 Oct 14;6(102):100652–100663. doi: 10.1039/C6RA17459K

Figure 3. HJC0123 induces LX-2 cell cycle arrest and promotes apoptosis.

Figure 3

(A) LX-2 cells were treated with HJC0123 (1 μM) for 24 hours. Cells were stained with propidium iodide as described in Methods and analyzed by flow cytometry; HJC0123 treatment significantly increased S-phase arrest. (B, C) Apoptosis was assessed by immunofluorescence staining using Yo-Pro-1 and PI as markers of early and late apoptosis, respectively; Hoechst 3342 was used as nuclear stain. HJC0123 treatment (1 μM for 24 hours) significantly induced early and late apoptosis. Immunofluorescence results were quantified as described in Methods. (D,E) Fluorometric TUNEL assay showed increased cell death in a dose-dependent fashion. The results are representative of at least three independent experiments. Error bars indicate ± SE.