FIG. 6.

Rejoining of DSBs with 3′ phosphate termini. Oligonucleotide ligation was used to generate plasmids containing DSBs with 3′ overhangs of 4, 6, 8, and 13 bases terminating in 3′ hydroxyls (A) or 3′ phosphates (B). The tpp1Δ apn1Δ apn2Δ genotype is represented as taaΔ. Data are expressed as the ratio of Ade⁺ to Leu⁺ colonies as in Fig. 2.