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. 2005 Feb;25(3):1135–1145. doi: 10.1128/MCB.25.3.1135-1145.2005

FIG. 1.

FIG. 1.

Targeted deletion of Pten in muscle and general characterization of mckPten+/+ and mckPten−/− mice. (A) PCR analysis of Cre-mediated recombination of the Pten locus (Δ4-5, top) and genotyping for the Pten-loxP allele (middle) and cre (bottom) as described previously (2). Results obtained from genomic DNAs of soleus (S) and EDL (E) muscles and tail (T) are shown. (B) Absence of Pten expression in muscle. Western blots of expression of Pten in livers (L), epididymal fat (F), brains (B), and thigh muscles (M) of 6-month-old mckPten+/+ and mckPten−/− mice are shown (top). Soleus (S) and EDL (E) muscle types show the same degree of Pten deletion (bottom). (C) Immunohistochemistry of Pten staining in soleus muscles of 6-month-old mckPten+/+ and mckPten−/− mice (magnification, ×25). (D) Similar weights of soleus and EDL muscles from 6-month-old mckPten+/+ (closed bars) and mckPten−/− (open bars) mice on a high-fat diet (n = 8 to 10 muscles per group; P = NS). (E) Similar weight gains by mckPten+/+ (closed symbols) and mckPten−/− (open symbols) mice on a chow (circles) or high-fat (squares) diet (n = 10 to 15 mice per group; P = NS). (F) Similar epididymal fat pad weights from 6-month-old mckPten+/+ and mckPten−/− mice after a chow or high-fat diet (P = NS). Error bars indicate standard errors of the means.