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. 2005 Feb;25(3):1135–1145. doi: 10.1128/MCB.25.3.1135-1145.2005

FIG.7.

FIG.7.

Insulin-stimulated 2-deoxyglucose uptake into isolated soleus muscle and Akt phosphorylation in prolonged high-fat feeding. (A) Soleus (left panel) or EDL (right panel) muscles isolated from 15-month-old mice fed a high-fat diet were incubated with or without 2 mU of insulin per ml for 30 min. 2-Deoxyglucose uptake was determined over 20 min. Results are the means ± standard errors from five independent experiments. (B) Net insulin effect on 2-deoxyglucose uptake in both muscles. (C) Soleus (left panel) or EDL (right panel) muscles isolated from 15-month-old mice fed a high-fat diet were incubated with or without 2 mU of insulin per ml for 10 min. Lysates (30 μg) were resolved by SDS-10% PAGE and immunoblotted with anti-phospho-T308 Akt-antibody. Representative immunoblots are shown (upper panels). Immunoblots were scanned within the linear range and quantified with the NIH image computer software. The quantified values (lower panels) represent the means ± standard errors from five experiments. All values are expressed relative to basal Akt phosphorylation in mckPten+/− mice, which was assigned a value of 1. (D) Lysates (25 μg) of isolated muscles were resolved by SDS-10% PAGE and immunoblotted with anti-Akt. A representative immunoblot is shown.