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. 2017 May 2;8(3):287–300. doi: 10.14336/AD.2016.0920

Figure 2. Aging increased the plasma and the ischemic muscle sFlt1 levels.

Figure 2.

A) The ELISA showed that the aged mice had higher levels of circulating sFlt1 protein throughout the follow-up period (n=6-8). B) Quantitative real-time PCR showed that the aged mice had higher levels of sFlt1 gene throughout the follow-up period (n=6-8). Data are mean ± SEM. *P<0.05 vs. corresponding day 0; †P<0.05 vs. corresponding young mice during ischemia; two-way ANOVA and Bonferroni post hoc tests. C and D) Representative images and quantitative data shows that sFlt-1+ (including splice isoform sFlt-1 [77 kDa] and sFlt1 isoform 14 [82 kDa]) staining signal was markedly increased in the ischemic myofiber space of aged mice at postoperative day 4. E) Representative triple imunofluorescent images show that Flt-1 is expressed in endothelial cells as well as macrophages. Data are mean ± SEM (n=6). *P<0.05, †P<0.05 by one-way ANOVA and Tukey’s post hoc tests. NS indicates no significant. Scale bar, 50 μm.