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EMSA performed with bacterially expressed recombinant human His-ETR-3 and synthetic 5′-end-labeled RNA from the clones indicated. Clones 017 and 019 are from round 0; all others are from round 5. Clone 522 was the only clone from round 5 without a UG or UGUU motif. Zero, 100, or 1,000 ng of ETR-3 was added to each reaction mixture.
