Table 1.
Primer sequences for Notch signaling and GABAergic neuron marker
| Gene name | Direction | Sequence of Primer (5′ - 3′) | Length (mer) | GC % | Tm (°C) | Gene ID (Gene Bank) |
|---|---|---|---|---|---|---|
| RBPJ | Forward | TTGCTTACCTTCAGGCGTGTG | 21 | 52.4 | 63.5 | 679028 |
| Reverse | GCCCAATGAGTCTGCTGCAA | 20 | 55 | 64.8 | ||
| Mash1 | Forward | AGGCCCTACTGGGAATGGA | 19 | 57.9 | 61.8 | 64186 |
| Reverse | CCCTGTTGCTGAGAACATTGA | 21 | 47.6 | 61.2 | ||
| Hes1 | Forward | GGAGAGGCTGCCAAGGTTTT | 20 | 60 | 64.9 | 29577 |
| Reverse | AGGCGACACTGCGTTAGGA | 19 | 52.2 | 64.5 | ||
| NeuN | Forward | AAGCTGAATGGGACGATCGTAGAG | 24 | 50 | 65 | 287847 |
| Reverse | GCATAGAATTCGGGCCCATAGAC | 23 | 52.2 | 64.9 | ||
| GAD67 | Forward | GCTGGAAGGCATGGAAGGTTTTA | 23 | 50 | 64.4 | 24379 |
| Reverse | AATATCCCATCACCATCTTTATTTGACC | 28 | 52.2 | 63.8 | ||
| β-3 tubulin | Forward | CTCAGGGGCCTTTGGACATC | 20 | 51.4 | 64.5 | 246118 |
| Reverse | CAGGCAGTCGCAGTTTTCAC | 20 | 52.2 | 62 | ||
| NF-H | Forward | CTCCCAAAAATTCCCTCCATA | 21 | 57 | 63.5 | 24587 |
| Reverse | CTGTCACTCCTTCCGTCACC | 20 | 54.5 | 65.2 | ||
| GAPDH | Forward | GGCACAGTCAAGGCTGAGAATG | 22 | 54.5 | 64.4 | 24383 |
| Reverse | ATGGTGGTGAAGACGCCAGTA | 21 | 52.4 | 62.8 |