FIG. 5.

Flow cytometric analysis of the hematopoietic cells from Pias1 null mice and their wild-type littermates. (A) Normal T- and B-lymphoid development in Pias1 null mice. Cells from the thymus and spleen of 6-week-old mice were stained with anti-CD4 and anti-CD8 (thymus) or anti-B220 and anti-IgM (spleen) and analyzed by flow cytometry. The percentages of cells within defined regions are indicated. Data are representative of three separate analyses. (B) Enhanced granulopoiesis in Pias1 null mice. The same splenocytes as in panel A were stained with the granulocyte-specific marker Gr-1 followed by flow cytometric analysis. Data are representative of three separate analyses. (C) Increased expression of G-CSF in Pias1 null thymus. Total RNA from the thymuses of Pias1 null mice and their wild-type littermates (n = 6) was subjected to Q-PCR analyses.