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Interaction of Polι with PCNA in the yeast two-hybrid system
| AD fusion | DNA BD fusion | Mean β-galactosidase activity ± SD | Fold activation |
|---|---|---|---|
| GAL4 AD-PCNA | GAL4 BD | 0.03 ± 0.002 | 1 |
| GAL4 AD-PCNA | GAL4 BD-Polι (WTa) | 1.14 ± 0.05 | 38 |
| GAL4 AD-PCNA | GAL4 BD-Polι (A546A547) | 1.27 ± 0.03 | 42.3 |
| GAL4 AD-PCNA | GAL4 BD-Polι (A426A427A546A547) | 0.029 ± 0.003 | 0.97 |
WT, wild type.
