FIG. 7.

Paxillin interacts with and regulates LIMK. (A and B) Lysates were prepared from randomly growing or Fn-plated Cos7 cells that had been transfected with LIMK1 or LIMK2 (see Materials and Methods). Immunoblots were probed with antibody to paxillin, LIMK1, or HA. (C) Rescued or Pxl^−/− MEFs were subjected to a fibronectin-plating assay as described above. Filters were probed with antibody to phosphocofilin (upper blot) or reprobed with antibody to cofilin (lower blot). Quantitation (shown below the phosphocofilin blot) was done on a Li-Cor infrared imaging system.