Table 2.
Selected post-genomic studies of clade III nematodes [2].
| Species | Clade [4] | Host | Life stage(s) | Platform / Method | Accession | Reference |
|---|---|---|---|---|---|---|
| mRNAs | ||||||
| Anguillicola crassus | 8A | Eel | L2, adult female, adult male | Roche 454 GS FLX | SRA: SRP010313 | [64] |
| geographical isolates | Illumina GAIIx | SRA: SRP010313 | [65] | |||
| Ascaris suum | 8B | Swine | egg L3, liver L3, lung L3, L4, adult female, adult male | Illumina HiSeq2000 | SRA: SRP010159 | [40] |
| adult male tissues | Roche 454 | n/a | [38] | |||
| embryo, larvae, adult tissues | Illumina GAII | GEO: GSE26956 | [35] | |||
| embryo, larvae, adult tissues | Illumina GAII | GEO: GSE38470 | [37] | |||
| reproductive & non-reproductive adult tissues | Illumina HiSeq2000 | SRA: SRP013609 | [39] | |||
| Brugia malayi | 8B | Human | egg, microfilaria, L3, L4, adult female, adult male | Illumina GAII | AE: E-MTAB-811 | [44] |
| intra-mosquito host | Illumina GAII | GEO: GSE53664 | [45] | |||
| drug-treated adult female | Illumina MiSeq | SRA: SRP064921 | [47] | |||
| Brugia pahangi | 8B | Canine, Feline | microfilaria, L3 stages, L4, adult female, adult male | Illumina HiSeq2000 | ENA: ERP001375 | WTSI |
| Dirofilaria immitis | 8B | Canine | adult female, adult male | Illumina GAIIx | AE: E-MTAB-714 | [52] |
| adult | Illumina HiSeq2000 | SRA: SRA048975 | [53] | |||
| microfilaria, L3, L4, adult female, adult male | Illumina GAII | SRA: SRP048819 | [56] | |||
| adult tissues | Illumina GAII | GEO: GSE67894 | [54] | |||
| Litomosoides sigmodontis | 8B | Mouse | microfilaria, adult female, adult male | Roche 454 GS FLX | SRA: ERA011678 | [59] |
| Loa loa | 8B | Human | n/a | Illumina GAII | BP: PRJNA60555 | BI |
| Onchocerca flexuosa | 8B | Deer | mixed stages | Roche 454 GS FLX | BP: PRJNA65265 | [62] |
| Onchocerca volvulus | 8B | Human | microfilaria, L2, L3, adult female | Illumina HiSeq2000 | ENA: ERP001350 | WTSI |
| Toxocara canis | 8B | Canine, Human zoonosis | L3, adult female, adult male, adult tissues | Illumina GAII | SRA: SRP051082 | [43] |
| Small RNAs | ||||||
| Ascaris lumbricoides | 8B | Human | miRNAs from parasitic adult | Illumina Solexa | n/a | [36] |
| Ascaris suum | 8B | Swine | miRNAs from parasitic adult | Illumina Solexa | n/a | [36] |
| egg, early embryo, L1, L2, adult tissues | Illumina GAII | GEO: GSE26957 | [35] | |||
| Brugia malayi | 8B | Human | adult | Illumina MiSeq | GEO: GSE56651 | [23] |
| Brugia pahangi | 8B | Canine, Feline | L3, adult | Illumina GAII | SRA: SRP009892 | [46] |
| Dirofilaria immitis | 8B | Canine | adult | Illumina Solexa | GEO: GSE35646 | [55] |
| Proteins | ||||||
| Ascaris suum | 8B | Swine | ESP from egg L3, lung L3, L4 | W ESI Q-TOF Premier MS | [42] | |
| ESP, perienteric and uterine fluids from adult female | TF LTQ-Orbitrap Elite MS | [41] | ||||
| Brugia malayi | 8B | Human | ESP from adult | AB 4700 Proteomics Analyzer MS | [48] | |
| ESP from microfilaria, adult female, adult male | W Micromass Q-TOF micro MS | [66] | ||||
| ESP from microfilaria, infective L3, molting L3, adult female, adult male | linear ion trap-FT MS | [49] | ||||
| tissue from L3, adult female, adult male; blood-borne and uterine microfilaria | linear ion trap–FT MS | [50] | ||||
| adult female tissues | TFS LTQ-Orbitrap XL MS | [51] | ||||
| drug-treated adult female | n/a | [47] | ||||
| Brugia pahangi | 8B | Canine, Feline | adult somatic extracts | AB Proteomics 4700 MALDI-TOF-TOF Analyzer MS | [67] | |
| Dirofilaria immitis | 8B | Canine | ESP from adult | TF LTQ-FT Ultra Mass MS | [57] | |
| adult somatic & surface antigens | AB QSTAR-XL nanoESI-Q-TOF MS | [58] | ||||
| adult tissues | TFS QExactive MS | [54] | ||||
| Litomosoides sigmodontis | 8B | Mouse | ESP and tissue from adult female and adult male | TFS LTQ-Orbitrap Velos MS | [60] | |
| Loa loa | 8B | Human | urine from infected human | TFS LTQ-Orbitrap Velos MS | [61] | |
| Onchocerca flexuosa | 8B | Deer | adult lysates | TFS LTQ Orbitrap Discovery hybrid MS | [62] | |
| Onchocerca ochengi | 8B | Bovine | L3, adult female, adult male, uterine microfilaria | TFS LTQ-Orbitrap Velos MS | [63] | |
Abbreviations: Broad Institute (BI); European Bioinformatics Institute (EBI); EBI ArrayExpress (AE); EBI European Nucleotide Archive (ENA); excretory/secretory products (ESP); mass spectrometer (MS); matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF); National Center for Biotechnology Information (NCBI); NCBI BioProject (BP); NCBI Gene Expression Omnibus (GEO); NCBI Sequence Read Archive (SRA); Thermo-Fisher/ThermoFisher Scientific (TF/TFS); Waters (W); Wellcome Trust Sanger Institute (WTSI)