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. 2017 May 11;19(6):496–508. doi: 10.1016/j.neo.2017.04.004

Figure 1.

Figure 1

PDGFRα is up-regulated upon stromal PTEN deletion and is expressed in the stroma throughout normal mouse mammary gland development. A, Representative images and quantification for PDGFRα staining on mammary gland sections from Ptenfl/fl and Fsp-cre;Ptenfl/fl mice (≥3 images per mouse, 3 mice per genotype; Scale bars – 100 μm; Bars represent the mean ± S.E.M, *P < .05). B, Western blots for PDGFRα and phospho-AKT expression in MMFs isolated from Ptenfl/fl and Fsp-cre;Ptenfl/fl mice. C, qRT-PCR for Pten and Pdgfra mRNA in MMFs derived from Ptenfl/fl and Fsp-cre;Ptenfl/fl mice (bars represent the mean ± S.E.M. relative to Rpl4) D, Representative images and quantification for PDGFRα staining on FFPE mammary sections harvested from virgin wild-type FVB/N mice at postnatal day 2 (PN2), 3 week, 5 week, 8 week, and 12 week of age. Glands were also harvested at pregnancy (embryonic day 18.5) and lactation (day 2) (≥5 images taken per mammary gland section, 3 mice per time point; Scale bars – 100 μm; Bars represent the mean ± S.E.M).