Fig. 1.

Heparin regulated expression, activation and downstream signaling of c-Met. a and b The effect of heparin on expression and activation of c-Met was determined by western blot, following serum starvation, SK-HEP-1 cells were treated with heparin in a time (0, 15′, 30′, 1 h, 2 h, 3 h, 6 h, 12 h) and dose (0, 1, 10, 100 μg/ml) dependent manner. Calnexin was used as a loading control. c Immunofluorescence was performed to examine expression and localization of p-Met (Y-1234/1235 and c-Met after 10 μg/ml heparin treatment for 2 h. d and e p-ERK1/2 (Thr202/Tyr204), p-Akt Ser473, total ERK1/2, total Akt and PTEN protein levels were determined by western blot after heparin treatment (0, 1, 10, 100 μg/ml) for 2 h. Calnexin levels were used as loading control