Fig. 3.

Heparin increased migration, invasion of SK-HEP-1 cells and ERK1/2-EGR1-MMPs activation through c-Met activation. a and b The effect of heparin mediated migration and invasion of SK-HEP-1 cells were investigated when cells were treated with 10 μg/ml heparin in the presence and absence of SU11274. Migrated and invaded cells were determined by staining with eosin red and crystal violet. Data are presented as the mean ± SD from 10 independent fields of 3 independent experiments. *p < 0.05. c and d The effect of SU11274 on heparin induced c-Met (Y-1234/1235) and ERK1/2 (Thr202/Tyr204) phosphorylation was determined after western blot analysis of lysates from 10 μg/ml heparin treated or non-treated SK-HEP-1 cells incubated in the presence and absence of SU11274. e EGR1 protein level was determined by western blotting when cells were left untreated or treated with 10 μg/ml heparin for 2 h, with or without SU11274. Calnexin levels were used as loading control. f MMP-2, 9 activities were determined by gelatin zymography. After serum starvation, SK-HEP-1 cells were treated with 10 μg/ml heparin, in the presence and absence of SU11274, for 24 h and their growth medium were collected for performing the assay