Fig. 5.

Heparin regulated G2/M cycle arrest and cell cycle molecules’ expression. a and b The cells were grown in the absence or presence of 10 μg/ml heparin for 24 h and then stained with propidium iodide and analysed by flow cytometry. The cell cycle was analyzed by Modfit software. Results represent three or more independent experiments performed in triplicates. (Bars indicate standard error of the mean (SEM), asterisks indicate statistically significant differences between the indicated groups) *p < 0.05. c, d and e The effects of heparin on CDKs’ (CDK2, CDK4, CDK6), Cyclins’ (Cyc D1, Cyc E, Cyc A) and CDKIs’ (p21, p27) expressions were determined by western blotting following cells were treated with 10 μg/ml of heparin for 24 h