Figure 1.

Cytotoxicity of T-2 toxin and/or DON in the cartilage-derived cell line, C28/I2. Cells in 96-well plates were exposed to five dilutions of T-2 toxin (from 2.5 to 40 ng/ml) or DON (from 100to 1600 ng/ml), or a fixed constant ratio of the two mycotoxins (T-2 + DON, ratio = 1:40). After (a) 24 h, (b) 48 h and (c) 72 h of exposure, the cell viability was analyzed by the MTT assay. Each data point represents the mean ± SEM from three independent experiments with replicate samples, *p < 0.05 indicates significant differences between the mixture and T-2 toxin alone, ^#p < 0.05 represents significant differences between the mixture and DON alone.