Table 2.
Dose–effect relationship parameters and mean combination index (CI) values of T-2 toxin or DON alone or in combination on C28/I2 cells.
| Mycotoxin | Time (h) | Dose–effect parameters | CI values at the following effect levels | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Dm | m | r | IC10 | IC25 | IC50 | IC75 | IC90 | ||
| T-2 toxin | 24 | 38.52 | 0.65 | 0.9484 | |||||
| 48 | 21.29 | 0.64 | 0.9529 | ||||||
| 72 | 8.73 | 0.82 | 0.9283 | ||||||
| DON | 24 | 2142.89 | 0.91 | 0.9722 | |||||
| 48 | 1202.48 | 1.03 | 0.9905 | ||||||
| 72 | 761.61 | 1.24 | 0.9722 | ||||||
| T-2 + DON | 24 | 1847.50 | 0.78 | 0.9844 | 2.60 (Ant) | 2.23 (Ant) | 2.01 (Ant) | 1.92 (Ant) | 1.94 (Ant) |
| 48 | 816.32 | 0.79 | 0.9913 | 2.17 (Ant) | 1.78 (Ant) | 1.60 (Ant) | 1.59 (Ant) | 1.75 (Ant) | |
| 72 | 471.09 | 0.89 | 0.9849 | 1.92 (Ant) | 1.88 (Ant) | 1.92 (Ant) | 2.05 (Ant) | 2.29 (Ant) | |
The parameters m, Dm, and r are the slope, antilog of the x-intercept, and the linear correlation coefficient of the median-effect plot, which signifies the shape of the dose-effect curve, the potency (IC50), and the conformity of the data to the mass-action law, respectively. IC10, IC25, IC50, IC75, and IC90 are the doses required to inhibit proliferation by 10%, 25%, 50%, 75% and 90%, respectively. Ant indicates antagonistic effect.