Table 3.
Dose–effect relationship parameters and mean combination index (CI) values of T-2 toxin or DON alone or in combination on L-02 cells.
| Mycotoxin | Time (h) | Dose–effect parameters | CI values at the following effect levels | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Dm | m | r | IC10 | IC25 | IC50 | IC75 | IC90 | ||
| T-2 toxin | 24 | 16.49 | 0.60 | 0.9180 | |||||
| 48 | 8.60 | 0.49 | 0.9400 | ||||||
| 72 | 2.11 | 0.63 | 0.9706 | ||||||
| DON | 24 | 1194.16 | 1.22 | 0.9510 | |||||
| 48 | 728.91 | 1.03 | 0.9757 | ||||||
| 72 | 426.62 | 1.12 | 0.9555 | ||||||
| T-2 + DON | 24 | 785.86 | 0.66 | 0.9538 | 1.76 (Ant) | 1.67 (Ant) | 1.80 (Ant) | 2.37 (Ant) | 3.81 (Ant) |
| 48 | 352.80 | 0.52 | 0.9600 | 1.35 (Ant) | 1.30 (Ant) | 1.47 (Ant) | 2.21 (Ant) | 4.50 (Ant) | |
| 72 | 123.01 | 0.63 | 0.9706 | 2.41 (Ant) | 1.96 (Ant) | 1.70 (Ant) | 1.70 (Ant) | 2.13 (Ant) | |
The parameters m, Dm, and r are the slope, antilog of the x-intercept, and the linear correlation coefficient of the median-effect plot, which signifies the shape of the dose-effect curve, the potency (IC50), and the conformity of the data to the mass-action law, respectively. IC10, IC25, IC50, IC75, and IC90 are the doses required to inhibit proliferation by 10%, 25%, 50%, 75% and 90%, respectively. Ant indicates antagonistic effect.