Table 4.
Dose–effect relationship parameters and mean combination index (CI) values of T-2 toxin or DON alone or in combination on HK-2 cells.
| Mycotoxin | Time (h) | Dose–effect parameters | CI values at the following effect levels | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Dm | m | r | IC10 | IC25 | IC50 | IC75 | IC90 | ||
| T-2 toxin | 24 | 55.48 | 0.80 | 0.9921 | |||||
| 48 | 16.19 | 0.80 | 0.9802 | ||||||
| 72 | 8.57 | 1.04 | 0.9472 | ||||||
| DON | 24 | 1754.26 | 0.61 | 0.9463 | |||||
| 48 | 381.38 | 0.56 | 0.9666 | ||||||
| 72 | 183.31 | 0.74 | 0.9753 | ||||||
| T-2 + DON | 24 | 1623.01 | 0.63 | 0.9906 | 1.37 (Ant) | 1.46 (Ant) | 1.62 (Ant) | 1.88 (Ant) | 2.28 (Ant) |
| 48 | 412.02 | 0.54 | 0.9643 | 1.13 (Ant) | 1.33 (Ant) | 1.67 (Ant) | 2.29 (Ant) | 3.41 (Ant) | |
| 72 | 157.68 | 0.69 | 0.9451 | 0.83 (Syn) | 1.01 (Ant) | 1.29 (Ant) | 1.71 (Ant) | 2.36 (Ant) | |
The parameters m, Dm, and r are the slope, antilog of the x-intercept, and the linear correlation coefficient of the median-effect plot, which signifies the shape of the dose-effect curve, the potency (IC50), and the conformity of the data to the mass-action law, respectively. IC10, IC25, IC50, IC75, and IC90 are the doses required to inhibit proliferation by 10%, 25%, 50%, 75% and 90%, respectively. Ant and Syn indicate antagonistic and synergistic effect, respectively.