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. 2017 May 22;7:2232. doi: 10.1038/s41598-017-01513-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Big brown bat c-Rel binds to the putative c-Rel binding site. We co-transfected plasmids expressing HA-tagged bat c-Rel and human/bat TNFα wildtype/mutant promoters in HEK293T cells. We immunoprecipitated c-Rel using HA-specific antibodies and quantified the amount of TNFα promoter pulled down by qRT-PCR. (a) Bat c-Rel pulled down a significantly (P = 0.021) higher amount of wildtype big brown bat TNFα promoter that contained the putative c-Rel binding motif over the mutant promoter in which the motif was deleted (mean ± SD, n = 4). (b) Bat c-Rel pulled down a significantly (P = 0.021) higher amount of mutant human TNFα promoter which contained the putative bat c-Rel binding motif over the human wildtype TNFα promoter, which lacked the c-Rel binding motif (mean ± SD, n = 4). qRT-PCR products obtained from quantifying the immunoprecipitated samples were electrophoresed on a gel and their representative cropped images are shown. Full length gel images are shown in Supplemental Fig. S5. Images have been acquired from two different gels. Statistical difference was calculated using two-tailed Mann Whitney U test for two independent samples *P < 0.05.