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. 2017 May 22;7:2232. doi: 10.1038/s41598-017-01513-w

Table 1.

siRNA, cloning and ChIP qRT-PCR primer sequences.

Sequence Primers Features and legend
Human TNF alpha promoter GCCGGTACCGCTGTCTGCTTGTGTGTGTG and GCCCTCGAGGGGGACACACAAGCATCAAG KpnI and XhoI sites
Big brown bat TNF alpha promoter GCCACGCGTAAGAATGTCTCGGGCTGTT and GCCCTCGAGGCTGTGTCTCCCAGAGGCC MluI and XhoI sites
Big brown bat cRel CDS cloned in pCMV-HA-N GCCGTCGACCATGCGTTTTCGATACAAATG and GCCGCGGCCGCTTACAAGTTAACCGGAAAAA SalI and NotI sites
CD.Ri.17417.13.1 (cRel_siRNA) - sequence rArArA rGrGrA rArGrC rUrArU rUrArU rUrUrC rArArG rArATA r = ribose sugar
CD.Ri.17417.13.1 (cRel_siRNA) - sequence2 rUrArU rUrCrU rUrGrA rArArU rArArU rArGrC rUrUrC rCrUrU rUrArC r = ribose sugar
CD.Ri.17417.13.2 (cRel_siRNA) - sequence rGrGrA rArGrA rUrUrC rArUrU rArArA rArArA rGrArA rUrCA A r = ribose sugar
CD.Ri.17417.13.2 (cRel_siRNA) - sequence2 rUrUrG rArUrU rCrUrU rUrUrU rUrArA rUrGrA rArUrC rUrUrC rCrUrU r = ribose sugar
Big brown bat TNF alpha promoter - ChIP primers GGCAGATGTGGCCACAGGCAGAG and CAGAGAGCTGAGTCCTTGACG
Human TNF alpha promoter - ChIP primers GGGGAGAACAAAAGGATAAGG and CTCTCACTTCTCAGGGCCCCAG

For siRNA sequences, ribonucleotides are preceeded by the letter ‘r’. Cloning primer sequences contain restriction sites as part of the sequence.