Table 1.
Sequence | Primers | Features and legend |
---|---|---|
Human TNF alpha promoter | GCCGGTACCGCTGTCTGCTTGTGTGTGTG and GCCCTCGAGGGGGACACACAAGCATCAAG | KpnI and XhoI sites |
Big brown bat TNF alpha promoter | GCCACGCGTAAGAATGTCTCGGGCTGTT and GCCCTCGAGGCTGTGTCTCCCAGAGGCC | MluI and XhoI sites |
Big brown bat cRel CDS cloned in pCMV-HA-N | GCCGTCGACCATGCGTTTTCGATACAAATG and GCCGCGGCCGCTTACAAGTTAACCGGAAAAA | SalI and NotI sites |
CD.Ri.17417.13.1 (cRel_siRNA) - sequence | rArArA rGrGrA rArGrC rUrArU rUrArU rUrUrC rArArG rArATA | r = ribose sugar |
CD.Ri.17417.13.1 (cRel_siRNA) - sequence2 | rUrArU rUrCrU rUrGrA rArArU rArArU rArGrC rUrUrC rCrUrU rUrArC | r = ribose sugar |
CD.Ri.17417.13.2 (cRel_siRNA) - sequence | rGrGrA rArGrA rUrUrC rArUrU rArArA rArArA rGrArA rUrCA A | r = ribose sugar |
CD.Ri.17417.13.2 (cRel_siRNA) - sequence2 | rUrUrG rArUrU rCrUrU rUrUrU rUrArA rUrGrA rArUrC rUrUrC rCrUrU | r = ribose sugar |
Big brown bat TNF alpha promoter - ChIP primers | GGCAGATGTGGCCACAGGCAGAG and CAGAGAGCTGAGTCCTTGACG | — |
Human TNF alpha promoter - ChIP primers | GGGGAGAACAAAAGGATAAGG and CTCTCACTTCTCAGGGCCCCAG | — |
For siRNA sequences, ribonucleotides are preceeded by the letter ‘r’. Cloning primer sequences contain restriction sites as part of the sequence.