Figure 3.

Induction of T cell stimulatory capacity and type 1 immune responsiveness by THP. (A) Immature human monocyte-derived DCs were stimulated with THP or LPS, washed extensively, irradiated, and subsequently cocultured with highly purified allogeneic T cells at the indicated ratios. After 48 hours, the cells were used as allogeneic stimulators as described above. DNA synthesis was assessed on day 5 and is expressed as mean counts per minute of a representative experiment. SD of triplicates were generally below 20%. Similar results were obtained in 4 other experiments. (B) For T cell cytokine production, immature DCs were stimulated with THP at the indicated concentrations or LPS, extensively washed, irradiated, and subsequently cocultured with highly purified allogeneic T cells at a DC/T cell ratio of 1:2. Cell-free supernatants were collected and analyzed by ELISA for IL-2 and IFN-γ secretion. Data are expressed as mean ± SEM of 4 different donor combinations.