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. 2017 May 22;7:2244. doi: 10.1038/s41598-017-01766-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Lenti-PB stable expression by lentivirus or PB transposition. (a) BFP flow cytometry analysis of virus packaging and production of Lenti-PB construct compared to the original lentiviral construct in mouse ES cells. No puromycin selection was added to these cells. (b) Graph indicating percent of BFP positive cells over ten-day time points of Lenti-PB plasmid co-transfected with PB transposase expressing plasmid (red) for stable integration or with p-bluescript plasmid (black) for transient expression. (c) Example of BFP positive mouse embryonic stem cells transfected with Lenti-PB.