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. 2017 May 22;7:2240. doi: 10.1038/s41598-017-02472-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The expression of sea bass IL-4/13 isoforms after in vitro stimulation with PHA and PMA. The mRNA levels of sea bass IL-4/13 isoforms were normalised to that of 18S rRNA in the same samples after real-time PCR analysis of HK and spleen leukocytes stimulated with L-15 medium (control) or with 10 µg/ml of PHA or with 1 µg/ml PMA for 4 h and 24 h, and expressed as arbitrary unit against the non-stimulated 0 h control. Data were expressed as the mean + SD. *p < 0.05 with respect to the time 0 control; ^**p < 0.01 with respect to the time 0 control; ***p < 0.001 with respect to the time 0 control; N = 4.